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What is chromatography?

Chromatography is a physical separation method at which the components to be separated are divided between two phases. One of the phases does not move and is called stationary phase, the other phase moves in a definite direction and is called mobile phase. According to the nature of the mobile phase we distinguish between gas-chromatography (GC), supercritical fluid chromatography (SFC) and high pressure liquid chromatography (HPLC).

Comparison between high pressure liquid chromatography (HPLC) and gas-chromatography (GC)

GC as well as HPLC are high pressure chromatographies. The most important difference lies however in the fact that for the GC only volatile matters are possible or matters that can be vaporized undecomposed at higher temperatures or of which we can produce reproducible volatile derivatives. Only about 20% of the known organic compounds can be analyzed gas chromatographically without pretreatment. Prerequisite for the fluid chromatography is that the sample can be dissolved in any solvent. Apart from cross-linked matters of high molecular weight this applies to all organic and the ionic inorganic matters.

Pressure units


Chromatography details